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Artigo Original

Transmission of mixed Plasmodium species and Plasmodium falciparum genotypes.

Am JTrop Med Hyg. 2003 Feb;68(2):161­8. PubMed PMID: 12641406.

44. Machado P, Manco L, Gomes C, Mendes C, Fernandes N, Salomé G, Sitoe L,

Chibute S, Langa J, Ribeiro L, Miranda J, Cano J, Pinto J,AmorimA, do RosárioVE,

Arez AP. Pyruvate kinase deficiency in sub­Saharan Africa: identification of a highly

frequent missense mutation (G829A;Glu277Lys) and association with malaria. PLoS

One. 2012;7(10):e47071. doi: 10.1371/journal.pone.0047071. Epub 2012 Oct 17.

PubMed PMID: 23082140; PubMed Central PMCID: PMC3474807.

45. Noormahomed EV, Orlov M, do Rosario V, Petersen BW, Guthrie C, Badaro

R, Schooley RT. A cross­sectional study of sub­clinical Plasmodium falciparum in-

fection in HIV­1 infected and uninfected populations in Mozambique, South­Eastern

Africa. Malar J. 2012 Aug 1;11:252. doi: 10.1186/1475­2875­11­252. P u b M e d

PMID:22853699; PubMed Central PMCID: PMC3468410.

46. Mendes C, Felix R, Sousa AM, Lamego J, Charlwood D, do Rosário VE, Pinto

J, Silveira H. Molecular evolution of the three short PGRPs of the malaria vectors

Anopheles gambiae and Anopheles arabiensis in East Africa. BMC Evol Biol. 2010

Jan 12;10:9. doi: 10.1186/1471­2148­10­9. PubMed PMID: 20067637; PubMed

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47. Marques PX, Saúte F, PintoVV, Cardoso S, Pinto J,Alonso PL, RosárioVE,Arez

AP. Plasmodium species mixed infections in two areas of Manhiça district, Mozambi-

que. Int J Biol Sci. 2005;1(3):96­102. Epub 2005 May 1. PubMed PMID: 16094461;

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48. Aranda C,Aponte JJ, Saute F, Casimiro S, Pinto J, Sousa C, RosarioVD, Petrarca

V, Dgedge M,Alonso P (2005). Entomological characteristics of malaria transmission

in Manhiça, a rural area in southern Mozambique. J Med Entomol. Mar,42(2):180­6.

PubMed PMID: 15799528.

49. Fernandes NE, Silveira H, Franco AS, Arez AP, Forte JM, do Rosário VE. De-

tection of malaria parasites in paraffin­embedded spleen and placental tissues by

nested PCR. Trans R Soc Trop Med Hyg. 2001 May­Jun;95(3):293­4. PubMed

PMID:11491000.

50. Fernandes

N, FigueiredoP, do Rosário VE, Cravo P.Analysis of sul-

phadoxine/pyrimethamine resistance­conferring mutationsof Plasmodium fal-

ciparum from Mozambique reveals the absence of the dihydrofolate reductase 164L

mutant. Malar J. 2007 Mar 23;6:35. PubMed PMID: 17378942; PubMed Central

PMCID: PMC1950477.

51. Lobo E, de Sousa B, Rosa S, Figueiredo P, Lobo L, Pateira S, Fernandes N, No-

gueira F. Prevalence of pfmdr1 alleles associated with artemether­lumefantrine tole-

rance/resistance in Maputo before and after the implementation of artemisinin­based

combination therapy.Malar J.2014Aug 6;13(1):300.doi:10.1186/1475­2875­13­300.

PubMed PMID: 25098280.

52. Salgueiro P,Vicente JL, Ferreira C,TeófiloV, Galvão A, do RosárioVE, CravoP,

Pinto J.Tracing the origins and signatures of selection of antifolate resistance in island

populations of Plasmodium falciparum. BMC Infect Dis. 2010 Jun 9;10:163. doi:

10.1186/1471­2334­10­163. PubMed PMID: 20534146; PubMed Central PMCID:

PMC2898820.

53. Cravo P, Figueiredo S, Nogueira F, Lopes D, Ferreira ID, Ferreira C, Gil JP,

do Rosario VE. High frequency of the genetic polymorphisms associated with

sulfadoxine­pyrimethamine resistance, among Plasmodium falciparum isolates from

SãoTomé and Príncipe,West Africa.AnnTrop Med Parasitol. 2004 Apr;98(3):293­6.

PubMed PMID: 15119975.

54. Loureiro LF, Cesário AM, Franco AS, Rosário VE. Malaria in São Tomé

and Principe: prevalenceanddrug­susceptibility.Ann TropMed Parasitol. 1 9 9 6

Apr,90(2):223­4. PubMed PMID: 8762414.

55. Lopes D, Nogueira F, Gil JP, Ferreira C, do Rosário VE, Cravo P. pfcrt and

pfmdr1 mutations and chloroquine resistance in Plasmodium falciparum from SãoTo-

mé and Príncipe,West Africa.AnnTrop Med Parasitol. 2002 Dec; 96(8):831­4. Pub-

Med PMID: 12625938.

56. Lee PW, Ji DD, Liu CT, Rampao HS, do RosarioVE, Lin IF, Shaio MF.Applica-

tion of loop­mediated isothermal amplification for malaria diagnosis during a follow­up

study in São Tomé. Malar J. 2012 Dec 6;11:408. doi: 10.1186/1475­2875­11­408.

PubMed PMID: 23217163; PubMed Central PMCID: PMC3528453.

57. Gonçalves L, Subtil A, de Oliveira MR, do RosárioV, Lee PW, Shaio MF. Baye-

sian Latent Class Models in malaria diagnosis. PLoS One. 2012;7(7):e40633. doi:

10.1371/journal.pone.0040633. Epub 2012 Jul 23. PubMed PMID: 22844405;

PubMed Central PMCID: PMC3402519.

58. Lee PW, Liu CT, do Rosario VE, de Sousa B, Rampao HS, Shaio MF. Potential

threat of malaria epidemics in a low transmission area, as exemplified by São Tomé

and Príncipe. Malar J. 2010 Sep 2;9:264. doi: 10.1186/1475­2875­9­264. PubMed

PMID:,20920216; PubMed Central PMCID: PMC2955676.

59. Culleton RL, Mita T, Ndounga M, Unger H, Cravo PV, Paganotti GM,

Takahashi N, Kaneko A, Eto H,Tinto H, Karema C, D’Alessandro U, do Rosário V,

Kobayakawa T, Ntoumi F, Carter R, Tanabe K. Failure to detect Plasmodium vivax

inWest and Central Africa by PCR species typing. Malar J. 2008 Sep 11;7:174. doi:

10.1186/1475­2875­7­174. PubMed PMID: 18783630; PubMed Central PMCID:

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60. Sutherland CJ, Tanomsing N, Nolder D, OguikeM,JennisonC, Pukrittayakamee

S, Dolecek C, HienTT, do RosárioVE,ArezAP, Pinto J, Michon P, EscalanteAA, Nosten

F, Burke M, Lee R, Blaze M, OttoTD, Barnwell JW, Pain A,Williams J,White NJ, Day

NP, Snounou G, Lockhart PJ, Chiodini PL, Imwong M, Polley SD.Two nonrecombining

sympatric forms of the human malaria parasite Plasmodium ovale occur globally. J Infect

Dis.2010 May 15;201(10):1544­50. doi:10.1086/652240.PubMed PMID: 20380562.

61. Ferreira ID, Lopes D, Martinelli A, Ferreira C, do Rosário VE, Cravo P. In vi-

tro assessment of artesunate, artemether and amodiaquine susceptibility and mole-

cular analysis of putative resistance­associated mutations of Plasmodium falciparum

from SãoTomé and Príncipe.Trop Med Int Health. 2007 Mar;12(3):353­62. PubMed

PMID: 17313506.

62. Ferreira ID, RosárioVE, Cravo PV. Real­time quantitative PCR with SYBR Green

I detection for estimating copy numbers of nine drug resistance candidate genes in

Plasmodium falciparum. Malar J. 2006 Jan 18;5:1. PubMed PMID: 16420686; Pub-

Med Central PMCID: PMC1363351.

63. Charlwood JD, Pinto J, Ferrara PR, Sousa CA, Ferreira C, GilV, Do RosárioVE.

Raised houses reduce mosquito bites. Malar J. 2003 Dec 10;2(1):45. PubMed PMID:

14667242; PubMed Central PMCID: PMC317347.

64. Charlwood JD,Alcântara J, Pinto J, Sousa CA, Rompão H, GilV, RosárioVE. Do

bednets reduce malaria transmission by exophagic mosquitoes?Trans R SocTrop Med

Hyg. 2005 Dec; 99(12):901­4. Epub 2005Aug 29. PubMed PMID: 16129466.

65. Pinto J, Donnelly MJ, Sousa CA, Malta­Vacas J, GilV, Ferreira C, PetrarcaV, do

RosárioVE, Charlwood JD.An island within an island: genetic differentiation ofAno-

pheles gambiae in SãoTomé,West Africa, and its relevance to malaria vector control.

Heredity (Edinb). 2003 Oct;91(4):407­14. PubMed PMID: 14512957.

66. Hagmann R, Charlwood JD, Gil V, Ferreira C, do RosárioV, Smith TA. Malaria

and its possible control on the island of Príncipe. Malar J. 2003 Jun 18;2:15. Epub

2003 Jun 18. PubMed PMID: 12875660; PubMed Central PMCID: PMC166171.

67. Moraes MF, Soares M, Arroz MJ, Do Rosário VE, Da Graça JP, Abecasis P.

[New concepts in hyperactive malarial splenomegaly]. Acta Med Port. 2003 Jan­

Feb;16(1):41­6. Portuguese. PubMed PMID: 12828005.

68. Lopes D, Rungsihirunrat K, Nogueira F, Seugorn A, Gil JP, do RosárioVE, Cra-

vo P. Molecular characterisation of drug­resistant Plasmodium falciparum from

Thailand. Malar J. 2002 Oct 14;1:12. Epub 2002 Oct 14. PubMed PMID: 12423551;

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69. de Almeida A, Arez AP, Cravo PV, do Rosário VE. Analysis of genetic muta-

tions associated with anti­malarial drug resistance in Plasmodium falciparum from the

Democratic Republic of East Timor. Malar J. 2009 Apr 9;8:59. doi: 10.1186/1475­

2875­8­59. PubMed PMID: 19358729; PubMed Central PMCID: PMC2672086.

70. de Almeida A, RosárioVE, Henriques G,Arez AP, Cravo P. Plasmodium vivax in

the Democratic Republic of EastTimor: Parasite prevalence and antifolate resistance­

associated mutations. Acta Trop. 2010 Sep;115(3):288­92. doi: 10.1016/j.ac-

tatropica.2010.04.008. Epub 2010Apr 20. PubMed PMID: 20412783.

71. Restrepo­Pineda E, Arango E, Maestre A, Do Rosário VE, Cravo P. Studies on

antimalarial drug susceptibility in Colombia, in relation to Pfmdr1 and Pfcrt. Pa-

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